- Classification: chemical auxiliary agent
- Appearance: white or slightly yellow powder
- CAS No.:9003-05-428
- Type: cationic
- Formula: (C3h5no)N
- Solid Content: 89% Min
- Application:swimming pool water treatment
- Transport Package: 25kg kraft paper bag
- Delivery: 5-15days after deposit
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- What is polyacrylamide gel electrophoresis?
- The most commonly used polyacrylamide gel electrophoresis for quantitative protein analysis is Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The polyacrylamide gel forms by polymerizing acrylamide and a crosslinking agent, i.e., N, N’-methylene-bis-acrylamide.
- What parameters characterize polyacrylamide gel?
- Two parameters characterizes polyacrylamide gel: total monomer concentration (% T, in g/100 ml) and weight percentage of crosslinker (%C). Deferring these two parameters, helps in regulation in the pore size of the gel to yield the best separation result. %T indicates the pore size of the prepared gel.
- How does polyacrylamide gel form?
- The polyacrylamide gel forms by polymerizing acrylamide and a crosslinking agent, i.e., N, N’-methylene-bis-acrylamide. It does not react with proteins and consists of pores and channels that allow the protein to move through it.
- What is the function of polyacrylamide gel?
- Polyacrylamide gel: It is the matrix that helps to separate proteins based on their size. It can be either prepared in the lab or can be purchased. Running buffer: It varies based on sample type. Its primary function is to allow the conduction of current across the gel.