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Classification: chemical auxiliary agent
Appearance: white crystal
CAS No.:9003-05-8369
Type: anionic,cationic,nonionic
Formula: (C3h5no)N
Solid Content: ≥87.5%
Application：water treatment / paper / textile / oil / food fac
Transport Package: 900-1000kg packed in one pallet
Delivery: prompt shipment

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How does sulfo sanpah react with polyacrylamide?
Sulfo‐SANPAH contains a phenylazide group at one end that reacts nonspecifically with polyacrylamide on photoactivation, and a sulfosuccinimidyl group at the other end that reacts constitutively with primary amines ( Fig. 3 ). Fig. 3. The heterobifunctional photoreactive reagent sulfo‐SANPAH.
What is sulfo sanpah used for?
Generally, the process involves functionalizing a glass surface (e.g., coverslips or multi wells) to facilitate polyacrylamide attachment during polymerization. Sulfo-SANPAH is used to conjugate an extracellular matrix protein of choice to the polyacrylamide gel for cell attachment.
When was polyacrylamide used as a substrate?
Polyacrylamide was used as a substrate for cultured cells as much as 30 years ago when hepatocytes were grown on nonadherent polyacrylamide gels ( Schnaar et al., 1978 ). In this early work, the polyacrylamide substrates were not used for mechanical studies but to generate a flat matrix for immobilizing sugars.
How do you remove a polyacrylamide gel from a culture chamber?
This will allow the gel to fit on top of the bottom gel unimpeded by the sides of the culture chamber, thus maximizing the contact between the two substrates. Extraneous medium on the top and bottom polyacrylamide gels is removed by aspiration with a Pasteur pipette.