- Classification: chemical auxiliary agent
- Appearance: white granule
- CAS No.:9003-05-6861
- Type: anionic
- Formula: (C3h5no)N
- Solid Content: ≥88.9%
- Application:paper chemicals, rubber auxiliary agents
- Transport Package: 25kg woven bag with pe inner
- Delivery: 15day
water treatment chemicals: cationic-polyacrylamide (cpam) for low cost
water treatment chemicals: cationic-polyacrylamide (cpam) for sale. sds page or sodium dodecyl sulphate-polyacrylamide gel electrophoresis is a technique used for the separation of proteins based on their molecular weight. it is a technique widely used in forensics, genetics, biotechnology and molecular biology
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- What is SDS polyacrylamide gel electrophoresis (SDS-PAGE)?
- SDS polyacrylamide gel electrophoresis (SDS-PAGE) involves the separation of proteins based on their size. By heating the sample under denaturing and reducing conditions, proteins become unfolded and coated with SDS detergent molecules, acquiring a high net negative charge that is proportional to the length of the polypeptide chain.
- What is polyacrylamide gel electrophoresis?
- The most commonly used polyacrylamide gel electrophoresis for quantitative protein analysis is Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The polyacrylamide gel forms by polymerizing acrylamide and a crosslinking agent, i.e., N, N’-methylene-bis-acrylamide.
- What acrylamide concentration is used for SDS-PAGE gel?
- The concentration of acrylamide used for the gel depends on the size of the proteins to be analyzed. Low acrylamide concentrations are used to separate high molecular weight proteins, while high acrylamide concentrations are used to separate proteins of low molecular weight (see table Compositions and separation properties of SDS-PAGE gels ).
- How do I prepare acrylamide gel for SDS-PAGE?
- Boil for 7 min at 95°C, and then load samples immediately onto a gel for SDS-PAGE. Materials required IMPORTANT: Acrylamide is a potent neurotoxin and is absorbed through the skin. Take appropriate safety measures particularly when weighing solid acrylamide/bis-acrylamide, and also when working with the solutions and gels.
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