sulfo sanpah polyacrylamide on sale in tanzania

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• Classification: chemical auxiliary agent
• Appearance: white granule/power
• CAS No.:9003-05-7446
• Type: anionic
• Formula: (C3h5no)N
• Solid Content: 88.5% Min
• Application：building materials industries
• Transport Package: one 20’fcl load in 18-20mt for usual
• Delivery: 15day

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regulation of cell attachment, spreading, and migration

we attribute this difference to the deposition of sulfo-sanpah on the paa hydrogel surface, which disrupts fiber formation of collagen monomers due to the non-specific protein conjugation and the blockage of triple helixes [34], [35], [36]. we then quantify the average fluorescence intensity of the collagen coatings among different samples.

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a novel method to make viscoelastic polyacrylamide gels for low cost

cover the surface of the gel with the sulfo-sanpah solution and illuminate with a 320–365 nm uv light source for 15 min. properly activated sulfo-sanpah should change color from bright orange into darker, burnt orange. rinse 3 times with hepes: 50 mm ph 8.2. immerse the gels in the protein solution in hepes: 50 mm ph 8.2.

polyacrylamide hydrogels for cell mechanics: steps toward,in the initial design of the hydrogels, the photoactivatable heterobifunctional reagent sulfosuccinimidyl‐6‐(4′‐azido‐2′‐nitrophenylamino) hexanoate, known as sulfo‐sanpah, was used. sulfo‐sanpah contains a phenylazide group at one end that reacts nonspecifically with polyacrylamide on photoactivation, and a sulfosuccinimidyl

a novel method to make polyacrylamide gels with mechanical

cover the surface of each gel with 80 µl sulfo-sanpah solution (for an 18-mm diameter gel) and illuminate with uv light (320-365 nm) for 10-15 min under the hood. from this step forward, gels that will be used for cell seeding should be maintained under aseptic conditions.

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a large-format polyacrylamide gel with controllable matrix

(1) polyacrylamide is synthesized in a glass dish using a pdms ring and acrylic cover. (2) the crosslinker sulfo-sanpah is activated on the polyacrylamide with uv light and the excess is rinsed off. (3) extracellular matrix protein is diluted in buffer and incubated on the polyacrylamide to initiate conjugation.

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stembond hydrogels control the mechanical microenvironment

hydrogels without aha were activated with sulfo-sanpah (thermoscientific, ma,usa). 1 mg ml −1 (unless otherwise noted) sulfo-sanpah was dissolved in hepes, and activated by uv-light for up to 30

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