- Classification: chemical auxiliary agent
- Appearance: white powder
- CAS No.:9003-05-7421
- Type: cationic
- Formula: (C3h5no)N
- Solid Content: ≥91%
- Application：effluent disposal
- Transport Package: 25kg woven bag with pe inner
- Delivery: 15day
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- How does sulfo sanpah react with polyacrylamide?
- Sulfo‐SANPAH contains a phenylazide group at one end that reacts nonspecifically with polyacrylamide on photoactivation, and a sulfosuccinimidyl group at the other end that reacts constitutively with primary amines ( Fig. 3 ). Fig. 3. The heterobifunctional photoreactive reagent sulfo‐SANPAH.
- Does sulfo sanpah change color?
- Properly activated sulfo-SANPAH should change color from bright orange to a darker burnt orange or brown. CAUTION: Do not overexpose the gels to UV light and do not allow the gels to dry during irradiation. Rinse the gels 3-5 times with 100 µl 50 mM HEPES pH 8.2 until all the sulfo-SANPAH has been removed.
- What is sulfo sanpah used for?
- UV-activated sulfo-SANPAH enables binding of protein to the surface of the hydrogels. Sulfo-SANPAH-treated PAAm hydrogels have been successfully used, for example, to study the impact of substrate stiffness on regulating fate choices of mesenchymal stem cells 7, 8.
- How do you make sulfo sanpah gel?
- The larger the gel, the more protein solution is needed. Rinse with 100 µl PBS 3 times and store in PBS at 4°C until cell seeding, but not longer than a couple of days. Prepare 5 mM sulfo-SANPAH in 25% DMSO and 75% 50 mM HEPES pH 8.2 (first dissolve sulfo-SANPAH in DMSO, then add HEPES in MilliQ water).
