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the pore size in the gel is influenced by two variables: 1) acrylamide and bisacrylamide concentrations (% t) and 2) crosslinker concentration (% c) the size of the pores inside the gels reduces as the % t is raised. as a result, gels with a larger percentage are suited for separating small nanomolecules from one another.

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polyacrylamide gels are characterized by two parameters: total monomer concentration (%t, in g/100 ml) and weight percentage of crosslinker (%c). by varying these two parameters, the pore size of the gel can be optimized to yield the best separation and resolution for the proteins of interest.

apparent pore size of polyacrylamide gels: comparison of gels,the apparent gel pore radii calculated in this manner ranged from 21 nm in gels containing 10.5 %t, 5%c to 200 nm in gels with 4.6%t, 2%c; similar to the values observed for polyacrylamide gels cast and run in tris-borate-edta (tbe) buffer (holmes and stellwagen, electrophoresis 1991, 12, 612–619). hence, the effective pore size of

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the effective pore sizes of the gels were estimated from the gel concentration at which the mobility of a given dna molecule was reduced to one-half its mobility at zero gel concentration. the estimated pore radii ranged from approximately 130 nm for 3.5% gels to approximately 70 nm for 10.5% gels.

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what is the typical pore size distribution in 10%

the typical pore size distribution in 10% polyacrylamide gels used in can vary based on the specific conditions and compositions. studies have shown that the pore radii in acrylamide-based hydrogels range from 20.4 to 47.1 a, depending on factors like the presence of cationic comonomers, crosslinkers, and total monomer concentrations. additionally, the synthesis of nanostructured

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figure 1. pore-scale images of mpem transport and retention in porous media during phase 2 in exp. 1: (a) suspended and dispersed flow (4 mm from the inlet), (b) preferential flow in large pores and pore-throats (18 mm from the inlet), (c) transport in large pores and pore-throats (36 mm from the inlet), and (d) retention on pore-

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  • What is polyacrylamide gel electrophoresis?
  • The most commonly used polyacrylamide gel electrophoresis for quantitative protein analysis is Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The polyacrylamide gel forms by polymerizing acrylamide and a crosslinking agent, i.e., N, N’-methylene-bis-acrylamide.
  • What is the difference between agarose gel and polyacrylamide gel electrophoresis?
  • In polyacrylamide gel electrophoresis, polyacrylamide gel separates macromolecules, i.e., proteins of size five kDa to 250 kDa. Similarly, it can also isolate DNA of 5- 500 bp size. In agarose gel electrophoresis, agarose gel separates DNA, RNA, and protein. It can isolate DNA about 50-20,000 bp in size.
  • Why is polyacrylamide gel electrophoresis useful in HIV testing?
  • PAGE is helpful to analyze the size and number of polypeptide subunits. It is useful in HIV tests to separate HIV protein. Polyacrylamide gel electrophoresis consists of the following benefits:
  • What parameters characterize polyacrylamide gel?
  • Two parameters characterizes polyacrylamide gel: total monomer concentration (% T, in g/100 ml) and weight percentage of crosslinker (%C). Deferring these two parameters, helps in regulation in the pore size of the gel to yield the best separation result. %T indicates the pore size of the prepared gel.