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section vii. technical specifications,the supplier’s bid, including the technical and financial proposals, and all other documents/statements submitted (e.g. bidder’s response to clarifications on the bid), including corrections to the bid resulting from the procuring entity’s bid evaluation; the schedule of requirements; the technical specifications;

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*v.s green, department of agronomy, purdue university, west lafayette, in -1150; d.e. stott, usda-ars national soil erosion research laboratory, west lafayette, in -1196, usa.*corresponding author: . abstract soil degradation is a significant problem throughout the world. use of soil amendments, including anionic polyacrylamide (pam), is one of many options for

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  • Why does polyacrylamide gel have small pores?
  • Polyacrylamide gel with small pores helps to examine smaller molecules better since the small molecules can enter the pores and travel through the gel while large molecules get trapped at the pore openings. As with all forms of gel electrophoresis, molecules may be run in their native state, preserving the molecules' higher-order structure.
  • How are polyacrylamide gels characterized?
  • Polyacrylamide gels are characterized by two parameters: total monomer concentration (%T, in g/100 ml) and weight percentage of crosslinker (%C). By varying these two parameters, the pore size of the gel can be optimized to yield the best separation and resolution for the proteins of interest.
  • What does %t mean in polyacrylamide gel?
  • %T indicates the relative pore size of the resulting polyacrylamide gel: higher %T refers to a larger polymer-to-water ratio and on average smaller pores. The practical ranges for monomer concentration are stock solutions of 30-40%, with different ratios of acrylamide monomer to crosslinker.
  • How do you determine the pore diameter of polyacrylamide gels?
  • The average pore diameter of polyacrylamide gels is determined by the total concentration of acrylamides (% T with T = Total concentration of acrylamide and bisacrylamide) and the concentration of the cross-linker bisacrylamide (%C with C = bisacrylamide concentration).